标准摘要
[中文适用范围]： 本文件规定了从棉籽、棉叶和由此衍生的原材料中提取聚合酶链式反应（PCR）质量脱氧核糖核酸（DNA）的实验室的要求和建议，所提取的DNA应足以用于PCR分析。本文件适用于以下方面： a) 识别可提取PCR质量DNA的棉花原料； b) 规定从棉花及其衍生原料中有效提取DNA的方法； c) 规定用于PCR扩增DNA的棉花特异性标记物。 通过对棉籽、棉叶及其衍生原料进行分析所得到的PCR结果，只能表明该样品并非来源于纯转基因（GMO）棉花。对于转基因棉花与其他棉花的混合物，本文件无法检测出棉纤维及其衍生材料中的转基因成分。 本文件不适用于种子、棉包或加工后的织物和纱线的批量取样。有关推荐的取样方法，请参见ISO 6497标准。关于批量材料或基于棉花的产品取样的通用指南，可参考如ASTM D1441-12和CEN/TS 15568等标准。 [外文原描述]： This document specifies requirements and recommendations to laboratories that perform extraction of polymerase chain reaction (PCR) quality deoxyribonucleic acid (DNA) from cottonseed, cotton leaf and raw material derived therefrom, that is sufficient for the purpose of PCR analysis. This document is applicable to: a) identifying cotton raw material from which PCR quality DNA can be extracted; b) specifying a method for effective DNA extraction from cotton and cotton-derived raw materials; c) specifying the cotton-specific marker(s) to be used as controls for PCR amplification of DNA. A PCR result obtained from analysis of cottonseed, cotton leaf and to some extant raw materials derived therefrom can only indicate that it is not derived from pure genetically modified organism (GMO)-derived cotton. Admixtures of GMO-derived cotton cannot be detected for cotton fibre and cotton fibre-derived materials. This document does not apply to bulk sampling of the seed, bale or processed fabric and yarn. A recommended sampling method is given in ISO 6497. General guidance for the sampling of bulk materials or for cotton-based products is available in standards such as ASTM D1441-12 and CEN/TS 15568.
英文名称Molecular biomarkers — Detection of DNA in cotton used for textile production — Part 1: Extraction of DNA from cotton, cottonseed and raw materials derived therefrom