标准摘要
[中文适用范围]： 该方法包括用盐酸水解磨碎的测试部分，然后通过柱色谱法分离总赖氨酸并通过 570 nm 光谱测定法测定。 将第二磨碎的测试部分与2,4-二硝基氟苯的乙醇溶液在碱性介质中反应，纯化，然后用盐酸水解，通过柱色谱法分离不可用的赖氨酸，并通过570 nm光谱测定。 [外文原描述]： The method consists in hydrolysing with hydrochloric acid of a ground test portion, dollowed by separation of the total lysine by column chromatography and determination by spectrometry at 570 nm. Reaction of a second ground test portion with an ethanolic solution of 2,4-dinitrofluorobenzene in an alkaline medium, purification, then hydrolysis with hydrochloric acid, separation of the non-available lysine by column chromatography, and determination by spectrometry at 570 nm.
英文名称Animal feeding stuffs — Determination of available lysine