食品链微生物学 横断面法检测、计数和分群沙门氏菌 第4部分：聚合酶链反应（PCR）鉴定单相泰泽菌株（1,4,[5],12:i: ）

[中文适用范围]： 本文件规定了一种体外分子鉴别和区分单相变型沙门氏菌肠亚种希拉利相变型（1,4,[5],12:i:-）缺乏第二H相H:1,2的方法，该方法从分离培养物开始。该方法检测第一H相鞭毛蛋白群间区的特定DNA序列以识别沙门氏菌肠亚种希拉利相变型（以下简称沙门氏菌希拉利相变型），并检测与第二H相鞭杆抗原表达相关基因的特定DNA序列。 本文件适用于：——将待分析分离物在单相沙门氏菌希拉利相变型和另一种非希拉利相的沙门氏菌变型之间进行区分，该变型具有相同的抗原配方；——识别待分析分离物是单相沙门氏菌希拉利相变型或（双相）沙门氏菌希拉利相变型。本文件适用于从以下来源中对属于沙门氏菌属的纯培养物进行分析：——供人食用的产品；——供动物饲用的产品；——食品和饲料生产和处理环境中的环境样品；——初级生产阶段的样品。本文件也可用于其他领域以识别单相沙门氏菌希拉利相变型（例如环境、人类健康、动物健康）。 [外文原描述]： This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium ( 1 ,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression. The method is applicable for: — differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella non-Typhimurium serovar that has the same antigenic formula; — identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium. This document is applicable for the analysis of a pure culture belonging to the genus Salmonella , isolated from: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. This document can also be applied in other domains for identification of monophasic Salmonella Typhimurium (e.g. environmental, human health, animal health). NOTE This method has been validated in a method evaluation study and in an interlaboratory study with a large set of different strains (target and non-target strains), isolated from different sources (food products, animals, animal feed, primary production samples and humans). For detailed information on the validation, see Annex E.