动物饲料 游离棉酚和总棉酚的测定

[中文适用范围]： 本方法适用于棉籽及棉籽饼粕。 该方法包括在3-氨基丙烷-1-醇存在下提取化合物，或者用2-丙醇和己烷的混合物来测定游离棉酚，或者用二甲基甲酰胺来测定总棉酚。 使用苯胺转化为棉酚-二苯胺，并测量最大吸光度波长（435 至 445 nm 之间）的吸光度。 [外文原描述]： The method is applicable to cottonseed and cottonseed meals and cakes. The method consists in extracting of the compound in the presence of 3-aminopropane-1-ol, either with a mexture of 2-propanol and hexane for the determination of free gossypol or with dimethylformamide for the determination of total gossypol. Conversion to gossypol-dianiline using aniline, and measurement of the absorbance at the wavelength of maximum absorbance (between 435 and 445 nm).