食品和动物饲料微生物学 凝固酶阳性葡萄球菌（金黄色葡萄球菌和其他菌种）计数的水平方法 第1部分：使用 Baird-Par 的技术

[中文适用范围]： 本文件规定了凝固酶阳性葡萄球菌的水平计数方法，通过对固体培养基（Baird-Parker 培养基）[10] 在 34 °C 至 38 °C 有氧培养和凝固酶确认后获得的菌落进行计数。 本文件适用于： ——供人类消费的产品； ——用于动物饲养的产品； ——食品和饲料生产、处理领域的环境样品，以及 — 初级生产阶段的样品。 这种水平方法最初是为了检查属于食物链的所有样品而开发的。 由于食物链中的产品种类繁多，这种横向方法可能并不适合所有产品的每个细节。 然而，预计所需的修改将被最小化，以便它们不会导致与该水平方法的显着偏差。 根据本文件发布时可获得的信息，该方法不被认为（完全）适合于检测发酵产品或含有基于葡萄球菌（例如木糖葡萄球菌）的技术菌群的其他产品（例如由生奶和某些生肉制品制成的奶酪）可能被以下物质污染： ——在贝尔德-帕克琼脂培养基上形成非典型菌落的葡萄球菌； ——背景植物群可能会掩盖正在寻找的菌落。 尽管如此，本文件和 ISO 6888-2 都具有同等地位。 [外文原描述]： This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (Baird-Parker medium) [10] after aerobic incubation at 34 °C to 38 °C and coagulase confirmation. This document is applicable to: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production, handling, and — samples from the primary production stage. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on Staphylococcus spp (e.g. S. xylosus ) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by: — staphylococci forming atypical colonies on a Baird-Parker agar medium; — background flora that can obscure the colonies being sought. Nevertheless, both this document and ISO 6888-2 are given equivalent status.