食品和动物饲料的微生物学 凝血酶阳性葡萄球菌(金黄色葡萄球菌及其他种)计数的水平方法 第2部分: 兔血浆纤维蛋白原琼脂培养基技术

[中文适用范围]： 本文件规定了凝固酶阳性葡萄球菌计数的水平方法，通过对34℃至38℃有氧培养后固体培养基（兔血浆纤维蛋白原琼脂培养基）上获得的菌落进行计数（参见参考文献[10]）。 本文件适用于： ——供人类消费的产品； ——用于动物饲养的产品； ——食品和饲料生产和处理领域的环境样品； ——初级生产阶段的样品。 这种水平方法最初是为了检查属于食物链的所有样品而开发的。 由于食物链中的产品种类繁多，这种横向方法可能并不适合所有产品的每个细节。 然而，预计所需的修改将被最小化，以便它们不会导致与该水平方法的显着偏差。 根据本文件发布时可获得的信息，该方法不被认为（完全）适合于发酵产品或含有基于葡萄球菌属的技术菌群的其他产品的检查。 （例如木糖链球菌）（例如由生奶和某些生肉制品制成的奶酪）可能被以下物质污染： ——在贝尔德-帕克琼脂培养基上形成非典型菌落的葡萄球菌； ——背景植物群可能会掩盖正在寻找的菌落。 尽管如此，ISO 6888-1 和本文件都具有同等地位。 1 [外文原描述]： This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]). This document is applicable to: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. Based on the information available at the time of publication of this document, this method is particularly suitable for the examination of fermented products or other products containing technological microbiota based on Staphylococcus spp. (e.g. S. xylosus ) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by: — staphylococci forming atypical colonies on a Baird-Parker agar medium; — background microbiota that can obscure the colonies being sought. Nevertheless, both ISO 6888-1 and this document are given equivalent status.