食品和动物饲料的微生物学.蜡状芽孢杆菌计数的水平方法.30℃时菌落计数技术

[中文适用范围]： 本国际标准规定了在30℃下采用菌落计数技术计数活的假定蜡状芽孢杆菌的水平方法。 它适用于 — 用于人类消费和喂养动物的产品，以及 — 食品生产和食品处理领域的环境样品。 注：为了获得可行的测试方法，验证阶段仅限于 MYP 琼脂和溶血测试的典型方面。 因此，引入“推定”一词是为了承认这样一个事实，即确认阶段无法将蜡状芽孢杆菌与其他密切相关但不太常见的芽孢杆菌物种（例如炭疽芽孢杆菌、苏云金芽孢杆菌、芽孢杆菌）区分开来。 . weihenstephanensis，蕈状芽孢杆菌。 在怀疑存在炭疽芽孢杆菌的情况下，额外的动力测试可能有助于区分蜡样芽孢杆菌和炭疽芽孢杆菌。 [外文原描述]： ISO 7932:2004 specifies a horizontal method for the enumeration of viable Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. In order to have a practicable test method, the confirmatory stage has been restricted to the typical aspect on MYP agar and the haemolysis test. Thus the term presumptive has been introduced in order to acknowledge the fact that the confirmatory stage does not enable the distinction of B. cereus from other closely related but less commonly encountered Bacillus species, such as B. anthracis , B. thuringiensis , B. weihenstephanensis , B. mycoides . An additional motility test may help to differentiate B. cereus from B. anthracis in cases where the presence of the latter is suspected.