标准摘要
[中文适用范围]： 该方法的原理是用乙酸乙酯和氯仿（体积比3：2）的混合液提取棒曲霉素，提取物经硅胶柱过滤，通过双向薄层法进行定性和定量测定。 色谱法。 使用 3-甲基-2-苯并噻唑啉腙 (MBTH) 盐酸盐溶液使斑点显色。 检测限为 25 镕/升（基于 50 毫升即饮苹果汁）。 更精确的分析方法是HPLC。 [外文原描述]： The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.
英文名称Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content — Part 2: Method using thin-layer chromatography