水质 培养的微生物计数方法通用指南

[中文适用范围]： 本文件规定了水微生物检验的每种培养技术的通用操作的要求并提供了指导，特别是样品、培养基、通用设备和玻璃器皿的制备，除非具体标准中另有要求。 它还描述了可用于培养检测和计数的各种技术以及确定哪种技术合适的标准。 本文件主要针对细菌、酵母菌和霉菌的检查，但某些方面也适用于噬菌体、病毒和寄生虫。 它不包括不基于培养微生物的技术，例如聚合酶链式反应（PCR）方法。 [外文原描述]： This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate. This document is mainly intended for examinations for bacteria, yeasts and moulds, but some aspects are also applicable to bacteriophages, viruses and parasites. It excludes techniques not based on culturing microorganisms, such as polymerase chain reaction (PCR) methods.