标准摘要
[中文适用范围]： 该文件规定了测量和记录多孔生物活性陶瓷材料的细胞迁移能力应遵循的测试方法。 本文件不适用于具有低或无细胞粘附特性的多孔材料，例如合成聚合物和金属。 这些类型的材料需要更长的时间才能使细胞从培养基质有效转移和迁移到测试样本。 为了尽量减少细胞传代、细胞种类、培养基、胎牛血清等细胞培养耗材差异的影响，本方法采用各国临床广泛使用的多孔生物活性陶瓷作为参考材料计算相对迁移距离。 [外文原描述]： ISO 19090:2018 specifies the test method to be followed for measuring and documenting the cell migration ability of porous bioactive ceramic materials. ISO 19090:2018 is not applicable to porous materials that have low or no cell adhesion properties, for instance synthetic polymers and metals. These types of materials will require longer times to allow effective transfer and migration of cells from the cultured substrate to the test specimen. To minimize influences of cell passages, cell kinds, differences in cell culture consumables including culture medium and fetal bovine serum etc., the method uses a porous bioactive ceramics, which is clinically and widely used in each country, as a reference material for calculation of relative migration distance.
英文名称Tissue-engineered medical products — Bioactive ceramics — Method to measure cell migration in porous materials